Insertion junction: LMJ.RY0402.112419_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.112419
Chromosome:chromosome 16
Location:1018545
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g649150 (1 of 1) PF13414//PF13920 - TPR repeat (TPR_11) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACCCCGAGACGCCCCATGCAGATCATT
Internal bar code:AGGTGTAAGACGATTAGCGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2413
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTACCACGTCCCTGACT
Suggested primer 2:GGTGTGTCATGACCAGTTGC