| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.112447 |
| Chromosome: | chromosome 9 |
| Location: | 2963130 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387800 | FER1 | Pre-apoferritin; (1 of 2) K00522 - ferritin heavy chain (FTH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGATGGGGGTGAAGAACATGTGAGGG |
| Internal bar code: | GGTACTATCCGCTGTCCAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 98.674 |
| LEAP-Seq n confirming: | 1265 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCTATCTAACTGAAGCG |
| Suggested primer 2: | GCTGCAGGAGTACCAGAACC |