| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112489 |
| Chromosome: | chromosome 4 |
| Location: | 1712156 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212600 | (1 of 15) IPR029057 - Phosphoribosyltransferase-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACAATCGATCATGGACACATGCGCCAC |
| Internal bar code: | GCGGGCTCTCTGCTTATGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 97.4565 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTGTGTTCCGCTTCTG |
| Suggested primer 2: | CCTGCTCCTCTTGTGGTCTC |