Insertion junction: LMJ.RY0402.112489_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.112489
Chromosome:chromosome 4
Location:1712161
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g212600 (1 of 15) IPR029057 - Phosphoribosyltransferase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGGTAGCACTATACTGTAGTGACCTTG
Internal bar code:AGCCCACGATCTTCCAGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:802
LEAP-Seq percent confirming:99.7532
LEAP-Seq n confirming:4042
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTTGTGTTCCGCTTCTG
Suggested primer 2:CCTGCTCCTCTTGTGGTCTC