| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112506 |
| Chromosome: | chromosome 17 |
| Location: | 3368354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723650 | KAT1,ATO1 | (1 of 1) 2.3.1.16 - Acetyl-CoA C-acyltransferase / Beta-ketothiolase; 3-oxoacyl CoA thiolase/acetyl-CoA acyltransferase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACAGGATCAGGCCATTGCCTCACTGCT |
| Internal bar code: | TATGCTTGTCCGACCAGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 99.6768 |
| LEAP-Seq n confirming: | 5860 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTGTTGCCGTGAAGTCC |
| Suggested primer 2: | CACGAGAGTGGCATTTAGCA |