| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112510 |
| Chromosome: | chromosome 10 |
| Location: | 2374240 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435250 | NIC13 | (1 of 1) 6.3.5.1 - NAD(+) synthase (glutamine-hydrolyzing) / Nicotinamide adenine dinucleotide synthetase (glutamine); NAD+ synthase (glutamine-hydrolyzing) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACAAACGGTGCGCCCCGGTCTGCCCCT |
| Internal bar code: | TGTACTTGCAGGGAGTATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.6575 |
| LEAP-Seq n confirming: | 582 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCATTAGATGCTTGTTGC |
| Suggested primer 2: | GCTTTTGGGCAGTTGACAAT |