| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112582 |
| Chromosome: | chromosome 14 |
| Location: | 1676955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619200 | (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCATGCGACGTCCCTCCACAACGTCACT |
| Internal bar code: | AGGCGGCCTCGGGGACATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 92.6636 |
| LEAP-Seq n confirming: | 6429 |
| LEAP-Seq n nonconfirming: | 509 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGAATCAGCAGGGTA |
| Suggested primer 2: | TGTGTGTGTGTGTGGTACGG |