| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112591 |
| Chromosome: | chromosome 1 |
| Location: | 4275838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028650 | (1 of 726) IPR011009 - Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCAGTCCTTAGGGTTTTCGATAGGGGG |
| Internal bar code: | TGACAGCGACATGCTCGACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 99.8435 |
| LEAP-Seq n confirming: | 638 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCACCTACCTGGAAGT |
| Suggested primer 2: | CACCACCACTTGACATTTGC |