| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.112616 |
| Chromosome: | chromosome 10 |
| Location: | 4546056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452300 | (1 of 4) 2.1.1.62 - mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGAACCATACCTGTACGTCATCATACG |
| Internal bar code: | CCGACGTGCATATCGGCTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 99.8373 |
| LEAP-Seq n confirming: | 1841 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCTGTGCTAGGCCTCAA |
| Suggested primer 2: | GAGAACAAGGCGTCTTCCTG |