| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.112649 |
| Chromosome: | chromosome 14 |
| Location: | 3772354 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632450 | (1 of 2) PTHR21131:SF0 - SEMINAL FLUID PROTEIN 33A3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCGCCCTGCATCGCCGGCTTCCTGGAC |
| Internal bar code: | GGTCATGAAACGTAAATTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGAACATGGGGCAAACG |
| Suggested primer 2: | GTTGTGCCTGAGGAGAAAGC |