| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112658 |
| Chromosome: | chromosome 6 |
| Location: | 8124535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304913 | (1 of 14) PF01476 - LysM domain (LysM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACAGCCCCTTCCTGATGGCTGGCAGCA |
| Internal bar code: | GAATTCACGTGGTACGGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.8229 |
| LEAP-Seq n confirming: | 1691 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCACGTTCAAGGGAAG |
| Suggested primer 2: | ATCCGGGCTCATGTTAGTTG |