| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112700 |
| Chromosome: | chromosome 6 |
| Location: | 1142816 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257050 | GBS2 | Granule bound starch synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGTACACCCGACCCGCCCAACACATT |
| Internal bar code: | GTAGCTAATATGAGTTAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 99.5984 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTTAATGAGCCCAAGT |
| Suggested primer 2: | CACGTTCATGTCACCACCTC |