| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112711 |
| Chromosome: | chromosome 10 |
| Location: | 738886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422750 | MOT10 | Predicted protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCTAGTCCTCGAATGCAATCGTTCGAC |
| Internal bar code: | CAGGGACACGTCGGAAGTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.8446 |
| LEAP-Seq n confirming: | 3856 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTAGCCCCAAAGGTTCT |
| Suggested primer 2: | ATACGTTCCCCTGTCCACAC |