Insertion junction: LMJ.RY0402.112723_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.112723
Chromosome:chromosome 8
Location:1995202
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTTGGGGATGGTGTGTTTACAACCACA
Internal bar code:GGCGGTCGGTGGAGGCAGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:82.1398
LEAP-Seq n confirming:1927
LEAP-Seq n nonconfirming:419
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACAGGAGGGGTTGTGTG
Suggested primer 2:CACACAGACACACACTCCCC