Insertion junction: LMJ.RY0402.112797_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.112797
Chromosome:chromosome 1
Location:2051411
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g010950 (1 of 2) PF00023//PF12796//PF13920 - Ankyrin repeat (Ank) // Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCTGACGTGGTCACAGGACCTGGAGGA
Internal bar code:CTGCACGACGCGGCGGTGACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:81
LEAP-Seq percent confirming:57.5884
LEAP-Seq n confirming:277
LEAP-Seq n nonconfirming:204
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTGGTTCGAGTCAGGGT
Suggested primer 2:GTGTGGAAGGACGGGTCTG