| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112830 |
| Chromosome: | chromosome 1 |
| Location: | 553725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003150 | protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTGGCGTTCGGGTGGACGTTGTAACA |
| Internal bar code: | CATCGTCGCTGCTAAAAGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 99.6528 |
| LEAP-Seq n confirming: | 6601 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGTGATGACCGTTACCT |
| Suggested primer 2: | CACTGCAGAGTGCTATGGGA |