Insertion junction: LMJ.RY0402.112832_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.112832
Chromosome:chromosome 2
Location:2847110
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g094700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGATCCACAATTCATACTCTCCTGTGCG
Internal bar code:TGGGGGTTCCGGGTTGGGAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:475
LEAP-Seq percent confirming:99.9019
LEAP-Seq n confirming:2036
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCACGAGGAACCTATACC
Suggested primer 2:GCGGGCTTACCTTGATGTTA