| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112845 |
| Chromosome: | chromosome 17 |
| Location: | 5730266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739050 | (1 of 230) IPR017986 - WD40-repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGGCTGTGGCGGCAGTAGCAGCAGAC |
| Internal bar code: | TAGCGCACACTCTTGGCAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 82.1576 |
| LEAP-Seq n confirming: | 25786 |
| LEAP-Seq n nonconfirming: | 5600 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGAGAAAGCGGCTGTAG |
| Suggested primer 2: | CAGCTCCAACTTCTGCTTCC |