Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.112855 |
Chromosome: | chromosome 1 |
Location: | 6473158 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g046052 | (1 of 6) 2.7.11.21 - Polo kinase / Polo-like kinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGGTTTGGCGAGGGGCGGTGAGAAGC |
Internal bar code: | TGCCCGCCACTCTTCTACGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 269 |
LEAP-Seq percent confirming: | 99.4654 |
LEAP-Seq n confirming: | 10420 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGCAGGTTGGTATAGCGG |
Suggested primer 2: | GAGCCATGATGCTGCTGTC |