| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112885 |
| Chromosome: | chromosome 11 |
| Location: | 1923633 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468350 | (1 of 1) K00486 - kynurenine 3-monooxygenase (KMO) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTGGCCCTGGTGTTCCCCGTTCGCCA |
| Internal bar code: | AATGATATGAGATCCTGACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.7065 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACACCGACACACATC |
| Suggested primer 2: | CTCGTCTCCCTCTTGCAATC |