| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112913 |
| Chromosome: | chromosome 1 |
| Location: | 5202687 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036200 | (1 of 1) PF01477//PF07228 - PLAT/LH2 domain (PLAT) // Stage II sporulation protein E (SpoIIE) (SpoIIE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCATTGCTGGTGCTCAGCTGCTGGTGC |
| Internal bar code: | CAGAGAGACGCGGAACAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 99.7006 |
| LEAP-Seq n confirming: | 7993 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGGTTGTTGGACAACTT |
| Suggested primer 2: | ACACTTTACGTGTGCCCCTC |