Insertion junction: LMJ.RY0402.112954_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.112954
Chromosome:chromosome 9
Location:6560030
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g408051 (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCGCCCAGCCGACTACGCCCCCACCAA
Internal bar code:CGCTCGCTATTTAGTACTTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:10
LEAP-Seq percent confirming:81.25
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCAACATTAGCTCCGT
Suggested primer 2:ACCCACCATCATCACCATCT