| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112968 |
| Chromosome: | chromosome_17 |
| Location: | 1623051 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCAGGCGTATCGGGCAGCCAGGTGGATGG |
| Internal bar code: | AGCGCCTAGTTGGGTCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 99.5902 |
| LEAP-Seq n confirming: | 3159 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGCACAAGAAGAAGTC |
| Suggested primer 2: | AACTACTGGACCGGCAACAC |