| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112992 |
| Chromosome: | chromosome 12 |
| Location: | 3737538 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514950 | CGLD17,HEL53 | (1 of 1) PTHR12873//PTHR12873:SF0 - T7-LIKE MITOCHONDRIAL DNA HELICASE // TWINKLE PROTEIN, MITOCHONDRIAL; Conserved in the Green Lineage and Diatoms | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCCTATGTCGGGTAGATTAGGCAGAC |
| Internal bar code: | CTCCCCTGACAGGTGAGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 45.1852 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGAGGAGGAGGAGGA |
| Suggested primer 2: | CCTATCCATGGAAATCCCCT |