| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112996 |
| Chromosome: | chromosome 17 |
| Location: | 2141876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712700 | TNP45 | putative transposase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGGTTGCCACTTGGATGCATGGGTT |
| Internal bar code: | TGAGCGGCTGGGGTTTGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.1882 |
| LEAP-Seq n confirming: | 1955 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTACGTTGCAGGGGTCA |
| Suggested primer 2: | GGGTGAGAAGGTCACCAAGA |