Insertion junction: LMJ.RY0402.113002_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113002
Chromosome:chromosome 11
Location:2127640
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g469400 HIK4,HKR4,HIK (1 of 1) IPR001789//IPR011006//IPR013761 - Signal transduction response regulator, receiver domain // CheY-like superfamily // Sterile alpha motif/pointed domain; response regulator of two-component system 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGACGCGAGCAGCACGCCAGGCAGCTTT
Internal bar code:AGCCGAAGGCGCGACTTAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:266
LEAP-Seq percent confirming:98.8235
LEAP-Seq n confirming:168
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACTATGGGATTGCTGGACG
Suggested primer 2:GAGCCGAAGAACTAACGTGC