| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113014 |
| Chromosome: | chromosome 16 |
| Location: | 2853060 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663500 | RPN10 | (1 of 1) K03029 - 26S proteasome regulatory subunit N10 (PSMD4, RPN10); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCACTCTCAAGCGATTTTGCCACTCTG |
| Internal bar code: | TAAAAGAGAGCCTAGGGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.9354 |
| LEAP-Seq n confirming: | 1548 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTCTACGCACACCTTGA |
| Suggested primer 2: | TCCGCTCAGCCTAAAACTGT |