Insertion junction: LMJ.RY0402.113046_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113046
Chromosome:chromosome 7
Location:6221076
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356500 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTACTGACTCACATGCCGCGGCGCCACT
Internal bar code:CCAGACGAATCCGGGTATGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:255
LEAP-Seq percent confirming:99.5114
LEAP-Seq n confirming:5703
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACATAGCAAATTCCCGC
Suggested primer 2:GCTTCTGAGGACTCAGGGTG