| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113105 |
| Chromosome: | chromosome 12 |
| Location: | 8974973 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544000 | RSP5 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Radial Spoke Protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAACGCAGGGGGTGGGGGGCCTGCCTC |
| Internal bar code: | CCGCTGGACGAACTGCTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.5429 |
| LEAP-Seq n confirming: | 1960 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGACGTTCTCAAGCTCC |
| Suggested primer 2: | CCGCAGGCTCAACTTAGAAC |