Insertion junction: LMJ.RY0402.113110_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113110
Chromosome:chromosome 2
Location:734596
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g078476 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGCCACACTCGTGTCAAAAGGTTGGCG
Internal bar code:CTCCCGTATGGCAGGGGCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:543
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:977
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GACCGTGCATATCGTGTGAC
Suggested primer 2:AGTCAAGCCCATCAACATCC