| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113173 |
| Chromosome: | chromosome 6 |
| Location: | 834917 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254450 | (1 of 1) K10295 - F-box protein 9 (FBXO9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTCTGCTTGTGGCTTTCGTACGGTCC |
| Internal bar code: | GAGGTGTGTCATCAGGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.6312 |
| LEAP-Seq n confirming: | 3512 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCATCATCCCACTTCAGC |
| Suggested primer 2: | CTTCTTCCAGAAGTGGCAGG |