Insertion junction: LMJ.RY0402.113174_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113174
Chromosome:chromosome 3
Location:290449
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g144564 MMP13 Matrix metalloproteinase; (1 of 1) IPR008752//IPR024079 - Peptidase M11, gametolysin // Metallopeptidase, catalytic domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATGGCATTAGTGCGCACGACCCTCCCC
Internal bar code:GCCGGCGAGAAACCAAGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:472
LEAP-Seq percent confirming:99.9407
LEAP-Seq n confirming:1685
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TATATGTGGACAGCATCGCC
Suggested primer 2:GGCTCTTCTGTGTCATGCAA