| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113207 |
| Chromosome: | chromosome 17 |
| Location: | 2756452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718468 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCCCAGTCTTGAGGCTGGTGACCGTG |
| Internal bar code: | GCACACGCTACCCGCGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 99.7696 |
| LEAP-Seq n confirming: | 5196 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCAGACTTGCCATAATTG |
| Suggested primer 2: | ATAAGGACAGGAATGCACGC |