Insertion junction: LMJ.RY0402.113224_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113224
Chromosome:chromosome 3
Location:8322601
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g199000 PHOT,PHT1,PHOT1 (1 of 1) PTHR24351//PTHR24351:SF76 - RIBOSOMAL PROTEIN S6 KINASE // PROTEIN TWIN LOV 1; Phototropin 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATCTGGGTGGGGTGGGTGTGGGGTGGT
Internal bar code:GATTGAACAACTGGGCTGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:902
LEAP-Seq percent confirming:99.5115
LEAP-Seq n confirming:5093
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTTCAAGGGCATCAACT
Suggested primer 2:TGGTCCACAACTTCCAAACA