Insertion junction: LMJ.RY0402.113224_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.113224
Chromosome:chromosome 11
Location:2979768
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478240 (1 of 1) K12178 - COP9 signalosome complex subunit 4 (COPS4, CSN4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGTGGGTGGAGGGAAAGTGGGCAGGTGG
Internal bar code:GCAATTCTAAGCATAGTGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:99.7265
LEAP-Seq n confirming:17501
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTCGGTACCAATCCTTGC
Suggested primer 2:CAGGACGACGACTCTGTGAA