| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113225 |
| Chromosome: | chromosome 12 |
| Location: | 5922148 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534200 | (1 of 1) PTHR24365//PTHR24365:SF381 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCGCGCGCCATGCTGCCGTCGCGGTAG |
| Internal bar code: | TCGTCTTATCAGTAGTATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 99.177 |
| LEAP-Seq n confirming: | 241 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGAGCGGATAGTGGAG |
| Suggested primer 2: | ATGCTTCTGTGAATGGAGGC |