| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113232 |
| Chromosome: | chromosome 2 |
| Location: | 4214239 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACTCCGGCCCTTGCGCGGGGCAGAGCA |
| Internal bar code: | CAGGGTGCCCTAAGACGTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.21 |
| LEAP-Seq n confirming: | 6405 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAAGCACGAAAGCACAC |
| Suggested primer 2: | TTGAACAAACAGCGAGCAAC |