Insertion junction: LMJ.RY0402.113244_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113244
Chromosome:chromosome 7
Location:518711
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g316100 (1 of 1) PTHR21512//PTHR21512:SF5//PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9 // FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACCCCAACTTGTACTGACACCGCCACG
Internal bar code:CCGTCTGGTGCACGGGGTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:341
LEAP-Seq percent confirming:99.7947
LEAP-Seq n confirming:486
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TACTACCTCCACAAACCCGC
Suggested primer 2:AGCCCAGGACAACCCTAGTT