| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113276 |
| Chromosome: | chromosome 3 |
| Location: | 6415500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194450 | CGL24 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAGGGTCAGCAGGTTAGGGTGTGACAT |
| Internal bar code: | ACCGGTTCGAGCGCATGATGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 99.23 |
| LEAP-Seq n confirming: | 3866 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGGCGCATTTTTACCA |
| Suggested primer 2: | ACACACTGGGCGGTTTCTAC |