| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113282 |
| Chromosome: | chromosome 13 |
| Location: | 2265317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578850 | MOT27 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGGATGCGTTCTTGCTGCCATGCTTT |
| Internal bar code: | ACTTGGTTCGCTGTACCGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 97.9616 |
| LEAP-Seq n confirming: | 4902 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGAACTTCCACTTGACA |
| Suggested primer 2: | CTCCCAAACACAAACACACG |