| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113285 |
| Chromosome: | chromosome 11 |
| Location: | 3371700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480451 | PHC42 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGCGATTGCGGCCGATGCGGGCTCCG |
| Internal bar code: | GCGTACAGAGACTTGTGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.8491 |
| LEAP-Seq n confirming: | 3971 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAGCCTCCGTCACCTGAG |
| Suggested primer 2: | TGTCAAAGGTGAAGACGCTG |