Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.113345 |
Chromosome: | chromosome 13 |
Location: | 3628495 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g588600 | KIN1-1,KIN1A | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATTGACCGCGAGCTAGTGGGTTCTTG |
Internal bar code: | CATGCGTATTTTTAATTCGGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 498 |
LEAP-Seq percent confirming: | 99.1132 |
LEAP-Seq n confirming: | 3800 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAGCATACCAATGCACAG |
Suggested primer 2: | CTTGAATCATGGCTGGTGTG |