| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113377 |
| Chromosome: | chromosome 1 |
| Location: | 2644321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015451 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTGTCTGGGGTCCAGCACGGCGGTCC |
| Internal bar code: | GCGCAGAAGATCCGCTCTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 98.6441 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTGATGCTATGAAGC |
| Suggested primer 2: | TCGCCAGCTACTTGCTCATA |