Insertion junction: LMJ.RY0402.113377_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113377
Chromosome:chromosome_8
Location:4144499
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGATGGTCAGGGGCAGCGAGGGCTGTTCAA
Internal bar code:GCGGGACTCGTTGTGGCTTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:558
LEAP-Seq percent confirming:99.3106
LEAP-Seq n confirming:2449
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTCGGTATGCATGGAGT
Suggested primer 2:ATCCGCTTATACCATGCAGC