Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.113377 |
Chromosome: | chromosome_13 |
Location: | 3790040 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589800 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTGTAGAACGAGAGCGTCTTTCCACCTCC |
Internal bar code: | CTCCGCCTAGCCTTAACATTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 787 |
LEAP-Seq percent confirming: | 96.2093 |
LEAP-Seq n confirming: | 1269 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCATTGCTCGCCTTACAG |
Suggested primer 2: | GCCCCGACATCTTGTGTAGT |