| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113391 |
| Chromosome: | chromosome 10 |
| Location: | 4214818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGAGCGCCGCACCGACGGTAAGCACTG |
| Internal bar code: | TATGTCCGTTTCCAGGCTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.5643 |
| LEAP-Seq n confirming: | 5942 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGACTACATGCACGGT |
| Suggested primer 2: | CATCCTACAGCCACCCAACT |