| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113433 |
| Chromosome: | chromosome 3 |
| Location: | 3270751 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCCACACAAAACCAACTCAAGCTTCAA |
| Internal bar code: | ACTTGGGCATTGCTCTGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.7093 |
| LEAP-Seq n confirming: | 1029 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACACAGCATCCACAGCA |
| Suggested primer 2: | AGCCTGAAGGAAGGAAGGAG |