| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113450 |
| Chromosome: | chromosome 6 |
| Location: | 1754335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262000 | SNR5 | SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTACGATATGTGTTCGTCCCGAGGAGA |
| Internal bar code: | GATGCGGCTTCGTAGATCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.4424 |
| LEAP-Seq n confirming: | 1070 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACGGGTTAGTAATGGAT |
| Suggested primer 2: | AGGCGGGTATGACGTATCAG |