Insertion junction: LMJ.RY0402.113454_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113454
Chromosome:chromosome 10
Location:3764104
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g446650 HEL46,MER (1 of 1) K15271 - ATP-dependent DNA helicase HFM1/MER3 [EC:3.6.4.12] (HFM1, MER3); conserved protein with DEAD/DEAH box helicase domain CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGCTGCTTGTTCGCCGCCACCTGGCG
Internal bar code:ACGGCTCCAGGGGCCTGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:175
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACATCCAAATCAGGTGC
Suggested primer 2:AGATCTCGCTGGTGCTGATT