Insertion junction: LMJ.RY0402.113480_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113480
Chromosome:chromosome 13
Location:2806081
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g583050 Tic55-like5,TIC55-5,PAO8 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGTCACTTGCCCCTGCCTCCCTCCCCT
Internal bar code:CTCGAACGTATTTTTTGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:99.5122
LEAP-Seq n confirming:1836
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGGAGAAACATGTGAGCA
Suggested primer 2:CAGTATTCAGCTCGGTGCAA